Chilled canine semen is collected from a stud dog and immediately mixed with a special nutrient-rich extender solution, then gradually cooled to 4–5°C. The extender protects the sperm during the cooling process, slows metabolism, and provides nutrients to maintain viability. As a result, the semen can typically be stored and shipped for 3 to 10 days.
This method allows semen to be safely transported domestically or to nearby countries, enabling artificial insemination without moving the stud dog. It preserves genetic quality, reduces stress on the animals, and supports a more flexible and efficient breeding program.
Chilled canine semen offers numerous practical and biological advantages in modern breeding. It combines the safety of artificial insemination with the vitality of freshly collected semen.
The main benefits include:
• High conception rates: When handled and inseminated properly, sperm motility and fertility remain high, often approaching the success rate of natural mating.
• Reduced stress and travel risk: There is no need to transport the stud dog or bitch, greatly minimizing stress and the risk of infection.
• Access to superior genetics: Breeders can utilize top-quality sires from other regions or countries, expanding breeding opportunities.
• Improved biosecurity: With no direct contact between dogs, the risk of disease transmission is minimized.
• Flexible timing: The semen can be used for several days after collection, allowing precise insemination scheduling according to ovulation timing.
Chilled semen is a safe, efficient, and humane method that expands breeding potential while maintaining excellent reproductive success.
The preparation of chilled canine semen is carried out hygienically and precisely, usually by a veterinarian or reproduction specialist, to preserve semen quality and viability. Each stage is carefully managed to protect sperm and maintain fertility during storage and transport.
The general steps are as follows:
• Collection: The stud dog’s semen is collected, often with the aid of a female in heat, to stimulate libido and obtain a high-quality sample.
• Fractionation: Canine semen consists of three parts:
① First fraction (0.5–5 ml): Mainly prostatic fluid that cleanses and lubricates the urethra.
② Second fraction (0.5–8 ml): Milky, sperm-rich portion used for artificial insemination and evaluation.
③ Third fraction (1–80 ml): Clear prostatic fluid that helps transport sperm during natural mating but is not suitable for preservation.
Because the third fraction contains excess prostatic fluid that can reduce sperm viability, only the first and second fractions are collected.
• Extension: The collected semen is mixed with a specially formulated extender to protect it from “cold shock” and provide nutrients for energy.
• Cooling and Packaging: The semen is slowly cooled to 4–5°C and sealed in an insulated container with ice packs. This maintains the ideal temperature without freezing, allowing the semen to remain viable for several days and suitable for long-distance shipment.
WorldDogs Asia provides everything you need to successfully collect, prepare, and ship fresh chilled canine semen. From collection bags, extenders, syringes, and packaging materials to temperature-controlled insulated shipping boxes, we supply high-quality, veterinarian-tested products specifically designed for canine reproduction.
We also provide step-by-step explanations and technical reference materials — all available as PDF guides — covering each stage of the process: collection, extension, chilling, and transport. These resources ensure proper handling and help maintain the highest semen quality throughout shipment.
All products and kits are available for purchase on our AI Goods Product Page.
Whether you are a breeder, veterinarian, or kennel, WorldDogs Asia supports you with complete, professional solutions for safe and efficient chilled semen transport.